Raw Glycerol Based Medium for DHA and Lipids Production, Using the Marine Heterotrophic Microalga Crypthecodinium cohnii

Crude glycerol, a biodiesel industry byproduct, and corn steep liquor (CSL) derived from starch industry, were used as carbon nitrogen sources, respectively, for lipid production, using the heterotrophic microalga C. cohnii grown in bench bioreactor, batch culture. The maximum biomass concentration, content productivity attained 5.34 g/L, 24.6% (w/w Dry Cell Weight-DCW) 0.016 g L−1 h−1, respectively. Flow cytometry analysis was to evaluate impact of these substrates on microalgae cells. A high proportion intact cells with enzymatic (esterases) activity (>50%) present throughout cultivation time course. These results indicate that crude glycerol CSL can be medium formulation DHA production this microalga, which reduce process costs an expected 84%.